All public logs

Combined display of all available logs of Microbial Ecology and Evolution Lab Wiki. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 15:49, 25 May 2023 Tommy talk contribs created page Plate Colonies Using CTTSA (Created page with "1. Warm up plates you’re using to 32°C. 2. Warm up a heat block to 55°C and place sterile test tubes into it. Have an equal number of test tubes to plates you’re pouring. 3. Determine how many cells you want to plate to determine the volume of cells you add to the CTTSA later. If you want to be able to count colonies, usually try to have ~200 - ~500 cells be added to the CTTSA. 4. Melt CTTSA in the microwave. This usually takes 3-4 minutes, depending on the amount...")
• 12:00, 29 March 2023 Tommy talk contribs created page Transform competent E. coli cells (Created page with "Transform competent E. coli cells 1.Prepare for transformation by ensuring the following: Water bath is set to 42°C Vial of SOC medium is warmed to room temperature X-gal/Kan plates are warmed to 37°C One Shot® Chemically Competent E. coli are thawed on ice Tubes of competent cells will be shaken horizontally which means cells will come into contact with the cap of the tube. To maintain good sterile technique and prevent contamination, do not touch the insi...")
• 11:53, 29 March 2023 User account Tommy talk contribs was created