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• 21:37, 11 May 2023 Dcapcha talk contribs created page Western Blot (Created page with "==Goal== * To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host. ==Protocol== ===Day 1=== ====Induction and Overnight Incubation of Bacterial Culture==== '''Day 2: Obtaining Supernatant and Lysate Samples from Bacterial Culture''' '''Da...")
• 22:56, 16 December 2022 Dcapcha talk contribs created page Running SDS-PAGE Gels (Created page with "==Goal== *Goal: To run an SDS-PAGE gel with the end goal of visualizing the protein on the gel ==Pre-Protocol Questions== # Do you need to know the amount of protein (ug) to load, or does it not matter? If you need to know, you must run a Bradford on your protein samples # Do you know if you have enough loading buffer, running buffer, and gel stain (Coomassie, silver, etc.)? # Are you planning to do a Western blot with this SDS-PAGE gel? This protocol is strictly for vi...")
• 22:25, 16 December 2022 User account Dcapcha talk contribs was created