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1 June 2023
- 02:1302:13, 1 June 2023 diff hist +830 Western Blot No edit summary current
- 02:0102:01, 1 June 2023 diff hist +80 Western Blot No edit summary
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11 May 2023
- 22:4122:41, 11 May 2023 diff hist +927 Western Blot No edit summary
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- 21:3921:39, 11 May 2023 diff hist +138 Western Blot No edit summary
- 21:3721:37, 11 May 2023 diff hist +552 N Western Blot Created page with "==Goal== * To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host. ==Protocol== ===Day 1=== ====Induction and Overnight Incubation of Bacterial Culture==== '''Day 2: Obtaining Supernatant and Lysate Samples from Bacterial Culture''' '''Da..."
- 21:2721:27, 11 May 2023 diff hist +22 Main Page →General microbiology protocols
16 December 2022
- 23:0323:03, 16 December 2022 diff hist +122 Running SDS-PAGE Gels →Pre-Protocol Questions current
- 23:0023:00, 16 December 2022 diff hist +12 Running SDS-PAGE Gels No edit summary
- 22:5922:59, 16 December 2022 diff hist +25 Running SDS-PAGE Gels No edit summary
- 22:5622:56, 16 December 2022 diff hist −7 Running SDS-PAGE Gels →Goal
- 22:5622:56, 16 December 2022 diff hist +6,130 N Running SDS-PAGE Gels Created page with "==Goal== *Goal: To run an SDS-PAGE gel with the end goal of visualizing the protein on the gel ==Pre-Protocol Questions== # Do you need to know the amount of protein (ug) to load, or does it not matter? If you need to know, you must run a Bradford on your protein samples # Do you know if you have enough loading buffer, running buffer, and gel stain (Coomassie, silver, etc.)? # Are you planning to do a Western blot with this SDS-PAGE gel? This protocol is strictly for vi..."
- 22:3022:30, 16 December 2022 diff hist +32 Main Page No edit summary