Rabies

Rachamreddy Venkata Chandrasekhar Reddy, Alapati Krishna Satya , Kota Sri Naga Leela Surendra , Samir Kumar Rana, Bhaskaran Mohana Subramanian, Girish Kumar Sharma , Villuppanoor Alwar Srinivasan (2016). [https://pdfs.semanticscholar.org/af55/03f4244f373749b2c915b1c5a124a8dc0e63.pdf Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Detection of Rabies Virus]

• RT-PCR primers were created from the most conserved region of the N gene sequence which is universal known in all rabies viruses. These researchers reviewed the results from the RT-LAMP assay using multiple methods, gel electrophoresis, turbidity, and color change by HNB dye. The RT-LAMP assays success at detection and sensitivity were 100% comparable to RT-PCR.

Marcus Panning, Sigrid Baumgarte, Susanne Pfefferle, Tanja Maier, Andreas Martens, Christian Drosten 2010). Comparative Analysis of Rabies Virus Reverse Transcription-PCR and Virus Isolation Using Samples from a Patient Infected with Rabies Virus

• The researchers created primer not to target a specific strain of rabies but rather to cover a larger range of rabies strains. The results that the researcher were trying to obtain was if RT-PCR worked as efficiently as rabies tissue culture infection test (RTCIT) and mouse inoculation test (MIT). Their results showed that RT-PCR was more sensitive than both RTCIT and MIT in both mice and human trials. This seems to be an effective way to detect rabies both intravirtam and postmortem that shows high sensitivity.