Brucellosis sp.

LAMP

I.A. Bhat M. Mashooq D. Kumar R. Varshney R. Rathore (2018). [Development of probe‐based real‐time loop‐mediated isothermal amplification for detection of Brucella https://onlinelibrary.wiley.com/doi/full/10.1111/jam.13938]

• Brucella spp. Is an intracellular bacteria that causes brucellosis and livestock such as cattle, goats and pigs are the most vulnerable. The researchers developed a rapid and sensitive detections of Brucella by LAMP. The primers were designed to detect the Omp25 gene which is one of the major outer membrane proteins. The sensitivity of this assay was found to be 10-fold higher than Taqman-based PCR. When testing the specificity of the assay they found that there was no cross reaction with other pathogenic bacteria. After amplification the results were evaluated using three different detection methods. The results show that these as well as LAMP is comparable in both sensitivity and specificity as a detection method for Brucella.

PCR

William S. Probert, Kimmi N. Schrader, Nhi Y. Khuong, Susan L. Bystrom, and Margot H. Graves (2004). [Real-Time Multiplex PCR Assay for Detection of Brucella spp., B. abortus, and B. melitensis https://jcm.asm.org/content/jcm/42/3/1290.full.pdf]

• The researchers aimed to targe the omp2 and bcsP31 which encode for an outer membrane protein and an immunogenic protein precursor respectively. The researchers provided all the primers used and the results indicated that the sensitivity and specificity of the assay for Brucella spp., B. abortus, and B. melitensis were all close to 100%. The researchers did not see any cross reactivity. After looking at the analytical sensitivity of this RT-PCR assay the researchers concluded that this assay is a useful tool for the direct detection of Brucella in clinical specimens.