Bovine Tuberculosis

LAMP

Mudenda B. Hang’ombe, Chie Nakajima, Akihiro Ishii, Yukari Fukushima, Musso Munyeme, Wigganson Matandiko, Aaron S. Mweene, Yasuhiko Suzuki (2011). Rapid Detection of Mycobacterium tuberculosis Complex in Cattle and Lechwe (Kobus leche kafuensis) at the Slaughter House

• Typically the detection of TB in animals involves clinical examination, skin tests and gamma interferon immunoassay. Here they propose a LAMP assay for the detection of TB. The researchers collected samples from the slaughtered animals in a slaughterhouse. The animals were examined for gross lesions and the tissues and organs suspected of being infected were tested. The primers used in the study were used by other workers to detect MTC in human sputum samples. The LAMP assay was 100% positive in culture positive specimen. LAMP was found to have a sensitivity of 95.2%.

PCR

Cristina P. Araújo, Ana Luiza A.R. Osório, Klaudia S.G. Jorge, Carlos A.N. Ramos, Antonio F. Souza Filho, Carlos E.S. Vidal, Agueda P.C. Vargas, Eliana Roxo, Adalgiza S. Rocha, Philip N. Suffys, Antônio A. Fonseca, Júnior, Marcio R. Silva, José D. Barbosa Neto, Valíria D. Cerqueira, and Flábio R. Araújo (2014). Direct detection of Mycobacterium tuberculosiscomplex in bovine and bubaline tissues through nested-PCR

• Through nested-PCR the researchers were able to identify TB. The researchers collected a variety of strains of TB to assess the analytical specificity and sensitivity of this PCR assay. The primers were designed to amplify the rv2807 which is a gene that encodes for a hypothetical protein in the bacterium complex. When the researchers compared the 50 DNA sample isolates they found that 49 out of the 50 (98.8%) were positive by nested PCR. This was designed to be a rapid post-mortem diagnostic tool which is directly applicable to bovine clinical samples.