Bovine Pleuropneumonia

LAMP

Georg Mair, Edy M Vilei, Abel Wade, Joachim Frey and Hermann Unger (2013). Isothermal loop-mediated amplification (lamp) for diagnosis of contagious bovine pleuro-pneumonia

• Contagious Bovine Pleuropneumonia is the most important pulmonary disease of cattle on the African continent which causes severe economic loss. The researchers presented a rapid and sensitive diagnostic tool using LAMP which is applicable for field conditions. The researchers used crude samples of pulmonary/pleural fluids and serum/plasma by dilution. The primers were created to detect the a putative pro-lipoprotein from the strain PG1. The analytical sensitivity of LAMP was comparable to the diagnostic capabilities of RT-PCR or nested PCR.

PCR Christiane SchneeEmail author, Martin Heller, Jörg Jores, Herbert Tomaso and Heinrich Neubauer (2011). [https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-7-47 Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoidessubsp. mycoidesSC through experimental infection in cattle]

• The researchers created a probe-based real-time PCR technique using TaqMan. The primers were designed to target two independent portions, MSC_0136 and MSC_1046, which code for hypothetical transmembrane proteins. The results indicate that the assay detects 49 MmmSC strains from diverse region but didn’t amplify DNA from 82 isolated of 20 non-target species which confirms a specificity of 100%. This PCR assay shows its usefulness as a method of detection for CBPP.