Anaplasmosis

Kubelova, M., Mazancova, J., Siroky, P. 2012. Theileria, Babesia, and Anaplasma detected by PCR in ruminant herds at Bié Province, Angola

• Anaplasmosis caused by Anaplasma marginale was diagnosied via PCR. PCR primers targeted the msp4 gene which encodes a surface protein. This gene is highly conserved in A. marginale strains from China, Israel, and the African field samples tested in this study. PCR products were run on a gel to identify positive and negative results.

Giglioti, R., Bassetto, C.C., Okino, C.H. et al. 2019. Development of a loop-mediated isothermal amplification (LAMP) assay for the detection of Anaplasma marginale

• LAMP and qPCR were used to identify A. marginale in infected bovine samples. Both LAMP and qPCR primers targeted the msp1b gene which encodes a surface protein. qPCR required two primers and a probe sequence and results were visualized on a gel. LAMP required 6 primers and was visualized by adding SYBR Green I dye and by running a gel. Neither method exhibited cross-reactivity with the three other bacterial infection including one of the same genus. qPCR was found to be 10 times more sensitive than the LAMP assay.