Bovine Pleuropneumonia

LAMP

Georg Mair, Edy M Vilei, Abel Wade, Joachim Frey and Hermann Unger (2013). Isothermal loop-mediated amplification (lamp) for diagnosis of contagious bovine pleuro-pneumonia

• Contagious Bovine Pleuropneumonia is the most important pulmonary disease of cattle on the African continent which causes severe economic loss. The researchers presented a rapid and sensitive diagnostic tool using LAMP which is applicable for field conditions. The researchers used crude samples of pulmonary/pleural fluids and serum/plasma by dilution. The primers were created to detect the a putative pro-lipoprotein from the strain PG1. The analytical sensitivity of LAMP was comparable to the diagnostic capabilities of RT-PCR or nested PCR.

PCR