Rabies: Difference between revisions
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Rachamreddy Venkata Chandrasekhar Reddy, Alapati Krishna Satya , Kota Sri Naga Leela Surendra , Samir Kumar Rana, Bhaskaran Mohana Subramanian, Girish Kumar Sharma , Villuppanoor Alwar Srinivasan (2016). [https://pdfs.semanticscholar.org/af55/03f4244f373749b2c915b1c5a124a8dc0e63.pdf Reverse Transcription Loop-Mediated Isothermal Amplification | Rachamreddy Venkata Chandrasekhar Reddy, Alapati Krishna Satya , Kota Sri Naga Leela Surendra , Samir Kumar Rana, Bhaskaran Mohana Subramanian, Girish Kumar Sharma , Villuppanoor Alwar Srinivasan (2016). [https://pdfs.semanticscholar.org/af55/03f4244f373749b2c915b1c5a124a8dc0e63.pdf Reverse Transcription Loop-Mediated Isothermal Amplification | ||
(RT-LAMP) Assay for the Detection of Rabies Virus] | (RT-LAMP) Assay for the Detection of Rabies Virus] | ||
*RT-PCR primers were created from the most conserved region of the N gene sequence which is universal known in all rabies viruses. These researchers reviewed the results from the RT-LAMP assay using multiple methods, gel electrophoresis, turbidity, and color change by HNB dye. The RT-LAMP assays success at detection and sensitivity were 100% comparable to RT-PCR. | |||
Marcus Panning, Sigrid Baumgarte, Susanne Pfefferle, Tanja Maier, Andreas Martens, Christian Drosten 2010). [https://jcm.asm.org/content/48/8/2960 Comparative Analysis of Rabies Virus Reverse Transcription-PCR and Virus Isolation Using Samples from a Patient Infected with Rabies Virus] | Marcus Panning, Sigrid Baumgarte, Susanne Pfefferle, Tanja Maier, Andreas Martens, Christian Drosten 2010). [https://jcm.asm.org/content/48/8/2960 Comparative Analysis of Rabies Virus Reverse Transcription-PCR and Virus Isolation Using Samples from a Patient Infected with Rabies Virus] | ||
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Revision as of 20:49, 29 April 2019
Rachamreddy Venkata Chandrasekhar Reddy, Alapati Krishna Satya , Kota Sri Naga Leela Surendra , Samir Kumar Rana, Bhaskaran Mohana Subramanian, Girish Kumar Sharma , Villuppanoor Alwar Srinivasan (2016). [https://pdfs.semanticscholar.org/af55/03f4244f373749b2c915b1c5a124a8dc0e63.pdf Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Detection of Rabies Virus]
- RT-PCR primers were created from the most conserved region of the N gene sequence which is universal known in all rabies viruses. These researchers reviewed the results from the RT-LAMP assay using multiple methods, gel electrophoresis, turbidity, and color change by HNB dye. The RT-LAMP assays success at detection and sensitivity were 100% comparable to RT-PCR.
Marcus Panning, Sigrid Baumgarte, Susanne Pfefferle, Tanja Maier, Andreas Martens, Christian Drosten 2010). Comparative Analysis of Rabies Virus Reverse Transcription-PCR and Virus Isolation Using Samples from a Patient Infected with Rabies Virus