Cysticercosis: Difference between revisions
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PreDec2022>Gpindzola (Created page with "Sreedevi, C., Hafeez, M., Kumar, P.A. et al. 2012. [https://link.springer.com/article/10.1007%2Fs11250-011-9893-2 PCR test for detecting Taenia solium cysticercosis in pig car...") |
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Sreedevi, C., Hafeez, M., Kumar, P.A. et al. 2012. [https://link.springer.com/article/10.1007%2Fs11250-011-9893-2 PCR test for detecting Taenia solium cysticercosis in pig carcasses.] | Sreedevi, C., Hafeez, M., Kumar, P.A. et al. 2012. [https://link.springer.com/article/10.1007%2Fs11250-011-9893-2 PCR test for detecting Taenia solium cysticercosis in pig carcasses.] | ||
*Two PCR assays were developed to detect Taenia solium in pigs. The targeted genes were TBR (large subunit rRNA) and Cox1. Cox1 was 10 times more sensitive. Selectivity was not measured. Results were read on an agarose gel. | |||
Nkouawa A, Sako Y, Nakao M, Nakaya K, Ito A. 2008. [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620829/ Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species.] | |||
*LAMP was used for differential detection of three Taenia species. Two target genes, cox1 and clp, were chosen for the assays. Both were successful for sensitive and specific detection. Clp misidentified 2 species though whereas cox1 had 100% accuracy. Results were read on an agarose gel and by visual inspection for turbidity. | |||
Additional Relevant Sources: | |||
Nkouawa A, Sako Y, Li T, et al. 2010. [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2937673/ Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans.] | |||
Latest revision as of 12:56, 16 December 2022
Sreedevi, C., Hafeez, M., Kumar, P.A. et al. 2012. PCR test for detecting Taenia solium cysticercosis in pig carcasses.
- Two PCR assays were developed to detect Taenia solium in pigs. The targeted genes were TBR (large subunit rRNA) and Cox1. Cox1 was 10 times more sensitive. Selectivity was not measured. Results were read on an agarose gel.
Nkouawa A, Sako Y, Nakao M, Nakaya K, Ito A. 2008. Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species.
- LAMP was used for differential detection of three Taenia species. Two target genes, cox1 and clp, were chosen for the assays. Both were successful for sensitive and specific detection. Clp misidentified 2 species though whereas cox1 had 100% accuracy. Results were read on an agarose gel and by visual inspection for turbidity.
Additional Relevant Sources:
Nkouawa A, Sako Y, Li T, et al. 2010. Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans.