Serial Dilutions of Phage: Revision history

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19 March 2023

9 March 2023

  • curprev 14:0814:08, 9 March 2023Xiwei Huang talk contribs 565 bytes −1 No edit summary
  • curprev 14:0814:08, 9 March 2023Xiwei Huang talk contribs 566 bytes −2 No edit summary
  • curprev 14:0714:07, 9 March 2023Xiwei Huang talk contribs 568 bytes +568 Created page with "1. Label 8, microcentrifuge tubes with 10^-1, 10^-2,10^-3 all the way down to 10^-8 a. These will serve as our dilution tubes 2. Add 900 uL of LB broth to each tube 3. Vortex the phage stock. 4. To the first tube (10^-1), add 100 uL of phage stock and vortex 5. Change the pippette tip. 6. To the second tube (10^-2), take 100 uL of tube 1, add it to the next dilution, and vortex the second tube 5. Repeat step 5 and 6 down to the 10^-8 tube a. Double check yo..."