Rehydrating New Primers: Revision history

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14 November 2023

21 March 2023

  • curprev 10:1810:18, 21 March 2023Jcomstock talk contribs 1,417 bytes +1,417 Created page with "Follow this protocol to rehydrate lyophilized primers. 1. Place all primer vials into the centrifuge and spin at ~13k rpm for 1 min to make sure the primer is at the bottom of the tube and will not float out as you open the vial. 2. Check the label of the vial. It should contain the amount of oligo in nanomoles. To make a 100mM stock solution of your primer, add 10x the amount of nanomoles of primer in uL. So if you have 18.5 nmol of primer, add 185uL of PCR water to..."