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2 June 2023
| N 16:03 | Generating Frozen Stocks of Strains diffhist +1,034 Jcomstock talk contribs (Created page with "1. Obtain an overnight culture of M. xanthus during log growth phase. Label a cryovial (2mL) on the top with your strain name, and on the side with strain name, date, your initials, and any information such as resistance markers and/or fluorescence. 2. Spin down 1.5ml of culture in a microfuge tube at max speed for 1 min and pipet off supernatant. 3. To the same tube, add another 1.5mL of culture, spin at max speed for 1 min, and pipet off supernatant. 4. Resuspend...") | ||||
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15:51 | Main Page 2 changes history +113 [Jcomstock (2×)] | |||
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15:51 (cur | prev) +46 Jcomstock talk contribs (→Myxococcus xanthus protocols) | ||||
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10:12 (cur | prev) +67 Jcomstock talk contribs (→Myxococcus xanthus protocols) | ||||
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N 10:31 | M. xanthus genomic DNA extraction with Zymo (yellow) kit 3 changes history +2,581 [Jcomstock (3×)] | |||
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10:31 (cur | prev) +166 Jcomstock talk contribs | ||||
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10:30 (cur | prev) +1,642 Jcomstock talk contribs | ||||
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10:20 (cur | prev) +773 Jcomstock talk contribs (Created page with "1. Turn a heat block to 55C. 2. If using an overnight culture, spin down 1.5mL of cells in a microfuge tube. If using cells from a plate, scrape up some culture with a p1000 pipet tip and resuspend it in 500uL of TPM, PBS, or DNA elution buffer from the kit. Spin these cells down at max speed for 1 min to create a pellet. 3. Pour and pipet of supernatant, and resuspend pellet THOROUGHLY in 200uL of TPM, PBS, or DNA elution buffer. 4. Place tube in the freezer for 5...") | |||
1 June 2023
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02:13 | Western Blot 16 changes history +15,409 [Dcapcha (16×)] | |||
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