Plaque Assays with Soft Agar: Revision history

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8 January 2024

17 September 2023

  • curprev 10:5010:50, 17 September 2023EricMiller talk contribs 3,404 bytes +3,404 Created page with "==Preparing Soft Agar== The soft agar should be prepared first, likely at least an hour before you pour # Make sure the water bath has enough water and is warming to 42C # You will use about 4 mL of soft agar per plate, calculate total volume of agar needed accordingly * Always prep at least one extra plate # Loosen the cap of the soft agar bottle and microwave the bottle of agar until it is fully liquid: * Check for bubbling about every 15 seconds * Y..."