Transformation — non-competent E. coli: Revision history

16 December 2022

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26 October 2022

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17 April 2022

• curprev 15:3315:33, 17 April 2022‎ PreDec2022>LStygianZ‎ 926 bytes +6‎ No edit summary
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7 January 2022

• curprev 17:2117:21, 7 January 2022‎ PreDec2022>EricMiller‎ 753 bytes +67‎ No edit summary

6 January 2022

• curprev 14:4214:42, 6 January 2022‎ PreDec2022>EricMiller‎ 686 bytes +686‎ Created page with "# Chill an overnight culture of E. coli cells. # Transfer 1.0ml of chilled cells into a microcentrifuge tube. Spin for 10 minutes at 4,000 rpm. # Remove supernatant without disrupting the bacterial pellet. # Add 100ul ice cold 100mM CaCl2. Resuspend the cells via stirring, but not vortexing or pipetting up or down. # Add 100ng of plasmid to tube. Mix via stirring, but not vortexing or pipetting up or down. # Allow the cells to incubate for 40 minutes on ice. # Turn on s..."