Streptococcus mutans Transformation Media Recipe (SMUR)

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S. mutans Recombination (SMUR) Media Recipe:

Final Recipe:

For 45mL of SMUR Media:

  1. Add 30 mL of 1.5x SMUR Base
  2. Add 9.5 mL of Sterile MilliQ Water
  3. Add 4.5 mL of 10% Glucose
  4. Add 450 uL of 100x SMUR Metals
  5. Add 450 uL of 100x SMUR Unstables
  6. Add 90 uL of 500x SMUR Vitamins
  7. Vortex and store in 4˚C Fridge

Do not use for longer than 2 weeks, or if anything has precipitated.

Stock Solutions:

500mL 1.5x Base Media:

BM SMUR.png

pH to 6.95 to 7.05, then autoclave.

45mL 100x Metals Solution:

Metals SMUR.png Add MgCl2 last and make sure everything is dissolved before it is added. There will be an exothermic reaction and and color change upon addition of MgCl2. If there is trouble dissolving everything, heat water bath to 45˚C and heat solution while vortexing occasionally. Then filter sterilize.

40mL 500x Vitamins Solution:

V SMUR.png If there is trouble dissolving, heat water bath to 45˚C and heat solution while vortexing occasionally. Then filter sterilize.

40mL 100x Unstables Solution:

U SMUR.png If there is trouble dissolving, heat water bath to 37˚C and heat solution while vortexing occasionally. Then filter sterilize.

Do not use for longer than 2 weeks.

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