Preparing Sanger Sequencing (Eurofins): Revision history

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16 December 2022

17 June 2022

  • curprev 17:0817:08, 17 June 2022‎ PreDec2022>EricMiller‎ 1,855 bytes +56‎ →‎Only PCR product or Plasmid
  • curprev 16:5716:57, 17 June 2022‎ PreDec2022>EricMiller‎ 1,799 bytes +193‎ No edit summary
  • curprev 16:5516:55, 17 June 2022‎ PreDec2022>EricMiller‎ 1,606 bytes +1,606‎ Created page with "__FORCETOC__ ==Only PCR product or Plasmid== Used if Eurofins will be added a primer to the Sanger sequencing tube. *Add 10ul of DNA to a 1.5ml microcentrifuge tube. The concentration should be: PCR Products, Purified 0.100–0.300kb 10–20 ng/ul PCR Products, Purified 0.301–1.000kb 20–40 ng/ul PCR Products, Purified >1.000kb 40–60 ng/ul Plasmid, Prepared < 1 .000kb 30-60 ng/ul Plasmid, Prepared 1.0 << 6.0kb 60-150 ng/ul Plasmid, Prepared 6.0 <..."
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