Media Protocols: Revision history

29 May 2024

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• curprev 11:5811:58, 22 March 2023‎ Jcomstock talk contribs‎ 1,036 bytes +1,036‎ Created page with "CTTYE Media (For 1L) 1. Start with 700mL of water in a 2L flask with a stir bar. If not making plates, you can mix right in the graduated cylinder. 2. Add 10mL of Tris (pH 8.0), 10 mL of 0.8M MgSO4, 1 mL of 1M KPO4 (pH 7.6). 3. Mix in 10g of casitone (sometimes labeled as Casein Peptone), 2 g of yeast extract. 4. Transfer to graduated cylinder and add water to a final volume of 1L. 5. Transfer back to flask to remove stir bar. 5. Add 15g agar (if making plates). If mak..."