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• 12:15, 17 September 2023 EricMiller talk contribs created page Media and Passaging (Created page with "==Minimal Media Recipe== {| class="wikitable" style="margin:auto" |+ LeMaster-Richards Minimal Media (LeMaster and Richards, 1982) |- ! Final Volume !! Water !! 4x Minimal Salts !! 2M MgSO4 !! Trace Minerals (4000x) !! Glucose (if needed) !! |- |10ml || 7.5ml || 2.5ml || 10ul || 2.5ul || xxul |- |100ml || 75ml || 25ml || 100ul || 25ul || xxul |- |250ml || 180ml || 62.5ml || 250ul || 62.5ul || xxul |} *Glycerol should be added to a final concentration of xx% (or xx%...")