Streptococcus mutans Transformation

Day 1: Streaking Out Plates

1. Warm 1 BHI Plate
2. Triple streak out S. mutans from freezer
3. Allow overnight growth in a 37˚C 5% CO2 incubator

Day 2: Overnight Growth

1. Add 1500uL of BHI to a well of the 96-well plate
2. Add ~5 colonies of S. mutans to the BHI
3. Allow overnight growth in a 37˚C 5% CO2 incubator

Day 3: Transformations

1. Add 1500uL of SMUR to a well of the 96-well plate
   1. Have one well set aside for a negative control, no XIP or DNA to this well
2. Pipette up and down the overnight culture with 500uL. Immediately after add 15uL of the overnight into SMUR wells.

Day 4: Plating Transformations