Western Blot

Goal

To extract protein samples from E. coli, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of E. coli.

Protocol

Day 1

Induction and Overnight Incubation of E. coli Culture

1. Grab however many erlenmeyer flasks you need for the experiment (including one for the negative control)

2.

3.