Western Blot

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Revision as of 21:41, 11 May 2023 by Dcapcha (talk | contribs)
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Goal

To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host.

Protocol

Day 1

Induction and Overnight Incubation of Bacterial Culture

Day 2

Obtaining Supernatant and Lysate Samples from Bacterial Culture

Running an SDS-PAGE on Supernatant/Lysate Samples

Transferring the SDS-PAGE Gel to the Membrane

Day 3

Primary Antibody Staining (Overnight)

Day 4

Secondary Antibody Staining

Imaging the Blot