Western Blot
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Goal
To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host.
Protocol
Day 1
Induction and Overnight Incubation of Bacterial Culture
Day 2: Obtaining Supernatant and Lysate Samples from Bacterial Culture Day 3: Day 4: Induction and Overnight Incubation of Bacterial Culture