Serial Dilutions of Phage
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1. Label 8, microcentrifuge tubes with 10^-1, 10^-2,10^-3 all the way down to 10^-8
a. These will serve as our dilution tubes
2. Add 900 uL of LB broth to each tube
3. Vortex the phage stock.
4. To the first tube (10^-1), add 100 uL of phage stock and vortex
5. Change the pippette tip.
6. To the second tube (10^-2), take 100 uL of tube 1, add it to the next dilution, and vortex the second tube
5. Repeat step 5 and 6 down to the 10^-8 tube
a. Double check your work by making sure tubes 1-7 have 900 uL of liquid in them, and the last should have 1 mL