Zone of Inhibition Assay
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The night/ day before create liquid cultures of the two bacteria that wish to work with
In the hood:
- Heat bock set to 45 degrees
- Vortexer
- 10ul, 200ul, and 1000ul pipettor
- Auto pipettor
- 10ul, 200ul and 100ul pipet tips
- 24 well plate
Create a beaker of verkon
- Fill a beaker with 500ml of water
- Add two ‘john dear’ spoon full of Verkon
Zone of Inhibition 1. Set the heat block to 45 degrees 2. Add 8 empty 13mm tubes with caps to the block 3. Warm up the BHI agar in the science microwave a. Start at power level 6 b. Watch if starts to boil lower the power level c. Should take about 10 min 4. When all melted lave to cool for a little so it is not boiling a. To cool a little faster put in a cool water bath 5. Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles) Note: multiple plate can be made in advance, but don’t leave for longer then a week 6. Label plate