Working with Antibiotics
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Stock solution concentrations:
- Penicillin 100 mg/mL, solvent MQ water
- Tetracycline 50 mg/mL, solvent MQ water
- Erythromycin 50 mg/mL, solvent Ethanol
Final concentrations for plates:
Final concentrations for broth:
Col-0 Data
| Antibiotic | Appreviation | Stock Solution
Concentration |
Solvent | Stock Concentration to
Working Concentration |
Notes |
|---|---|---|---|---|---|
| Ampicillin | Amp | 100mg/ml | H2O | 1000x | |
| Carbenicillin | Crb | 100mg/ml | 50% Ethanol | 1000x | |
| Chloramphenicol | Cam | 20mg/ml | 100% Ethanol | 1000x | |
| Cycloheximide | Chx | 100mg/ml | DMSO | 1000x | Toxic to fungi and animals (including humans)
Use protective gear; work in chemical hood. |
| Erythromycin | Ery | 250mg/ml | 100% Ethanol | 1000x | |
| Kanamycin | Kan | 50mg/ml | H2O | 1000x | |
| Spectinomycin | Spec | 60mg/ml | H2O | 1000x | |
| Streptomycin | Str | 100mg/ml | H2O | 1000x | |
| Tetracycline | Tet | 10mg/ml | 70% Ethanol | 1000x | Light sensitive
Wrap tubes and plates in aluminum foil |