Peste Des Petits Ruminants

E Couacy-Hymann, F Roger, C Hurard, J.P Guillou, G Libeau, A Diallo. (2002). Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay need access.

• this assay was based on rapid purification of RNA on glass beads followed by RT-PCR. They used a set of primers created to target NP3/NP4 that encodes for the nucleocapsidase protein. The PCR was then detected using UV illumination after agarose gel electrophoresis or by hybridization using a oligonucleotide probe after blot transfer. They found that this technique was 1000-fold more sensitive than convention titration techniques.

C.Senthil, KumaraG.Dhinakar, RajaA.Thangaveluab, M.S.Shaila. (2007) [Performance of RT-PCR-ELISA for the detection of peste des petits ruminants virus https://www.sciencedirect.com/science/article/pii/S0921448806002458]