FMDV

Farooq, U., Latif, A., Ullah, A., Zahur, A.B., Khan, S.U.H., Ahmed, Z., Rodriguez, L.L., Smoliga, G. 2015. Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan

• Reverse transciptase loop-mediated isothermal amplification was used to detect three of the six serotypes of FMDV (O, A, and Asia-1). Three pairs of primers are required for each LAMP reaction. For all three serotypes the primers targeted the 3D polymerase gene (primer sequences included in methods). The reaction uses reverse transcriptase and DNA polymerase and runs at 60℃ for up to 1 hour. Presence of viral RNA was visualized with Hydroxy napthol blue which changes from purple to blue for a positive result. RT-LAMP was shown to be more sensitive than conventional PCR and comparable to real time PCR.