Blue Tongue

Maan S, Maan NS, Batra K, Kumar A, Gupta A, Rao PP, Hemadri D, Reddy YN, Guimera M, Belaganahalli MN, Mertens PP. (2016) “Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India” (ask for access to this paper)

• Reverse-transcription-loop-mediated-isothermal-amplification (RT-LAMP) assay were created to detect eastern or western topotype of BTV. They created primers that recognized 6-distinct sequences of BTV genome segment 1. These assays were able to detect BTV RNA in all positive isolates tested. The sensitivity of RT-LAMP is similar to the sensitivity of RT-PCR but higher than conventional RT-PCR. Could not detect other local viruses that cause symptoms similar to BTV.

Jung-Yong Yeh, Ji-Hye Lee, Hyun-Ji Seo, Jee-Yong Park, Jin-San Moon, In-Soo Cho, In-Soo Choi,Seung-Yong Park, Chang-Seon Song, Joong-Bok Lee (2011). [Simultaneous Detection of Rift Valley Fever, Bluetongue, Rinderpest, and Peste des Petits Ruminants Viruses by a Single-Tube Multiplex Reverse Transcriptase-PCR Assay Using a Dual-Priming Oligonucleotide System]

• using RT-PCR the researchers have created a one-step single-tube multiplex RT-PCR that is able to detect not only BTV but RVFV, RPV and PPRV. Using samples of whole blood or oral mucosal tissue they were able to detect these viruses. This multiplex RT-PCR had sensitivity that was 10-fold lower than that of the corresponding uniplex RT-PCR in oral mucosal homogenate suspension than in whole blood. would be a great choice for doing screen testing on animal in an outbreak because whole blood would typically be used over oral mucosal. much more cost effective than uniplex assays.