All public logs

Combined display of all available logs of Microbial Ecology and Evolution Lab Wiki. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 13:54, 25 February 2024 Jcomstock talk contribs created page Spore Assay (Created page with "1. Harvest overnight M. xanthus culture during log growth phase, wash twice in TPM, and concentrate to %x10^9 cells/ml. You will likely need several mL of culture to plate out a spore assay. Each large TPM plate will need 4 groups of 5 20uL spots (400uL per plate, where one plate = 1 replicate). You may wish to use the 15mL conical tubes and the centrifuge in superlab to prepare cells. 2. While you are washing and preparing cells, leave TPM plates open to dry by flame o...")