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• 12:41, 24 July 2023 EricMiller talk contribs created page Streptococcus mutans Transformation (Created page with "Day 1: Grow up a single colony of S. mutans (from BHI plate in fridge or incubator) in 3ml of of BHI, in 37 degree 5% CO2 incubator. Day 2: #Warm two tubes of BHI to 37 degrees; one with 3ml (for bacteria growth), and one with ~7ml (as a blank and extra in case the OD600 of the experimental one needs to be diluted down) in either the incubator or heat block. #Turn on Spectrometer #1:20 Dilution of overnight culture into the Exp BHI (from step 1) (150uL of the overnight...")