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• 16:03, 2 June 2023 Jcomstock talk contribs created page Generating Frozen Stocks of Strains (Created page with "1. Obtain an overnight culture of M. xanthus during log growth phase. Label a cryovial (2mL) on the top with your strain name, and on the side with strain name, date, your initials, and any information such as resistance markers and/or fluorescence. 2. Spin down 1.5ml of culture in a microfuge tube at max speed for 1 min and pipet off supernatant. 3. To the same tube, add another 1.5mL of culture, spin at max speed for 1 min, and pipet off supernatant. 4. Resuspend...")