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Combined display of all available logs of Microbial Ecology and Evolution Lab Wiki. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

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  • 10:20, 2 June 2023 Jcomstock talk contribs created page M. xanthus genomic DNA extraction with Zymo (yellow) kit (Created page with "1. Turn a heat block to 55C. 2. If using an overnight culture, spin down 1.5mL of cells in a microfuge tube. If using cells from a plate, scrape up some culture with a p1000 pipet tip and resuspend it in 500uL of TPM, PBS, or DNA elution buffer from the kit. Spin these cells down at max speed for 1 min to create a pellet. 3. Pour and pipet of supernatant, and resuspend pellet THOROUGHLY in 200uL of TPM, PBS, or DNA elution buffer. 4. Place tube in the freezer for 5...")