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• 15:49, 25 May 2023 Tommy talk contribs created page Plate Colonies Using CTTSA (Created page with "1. Warm up plates you’re using to 32°C. 2. Warm up a heat block to 55°C and place sterile test tubes into it. Have an equal number of test tubes to plates you’re pouring. 3. Determine how many cells you want to plate to determine the volume of cells you add to the CTTSA later. If you want to be able to count colonies, usually try to have ~200 - ~500 cells be added to the CTTSA. 4. Melt CTTSA in the microwave. This usually takes 3-4 minutes, depending on the amount...")