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• 12:06, 25 May 2023 Jcomstock talk contribs created page Development Assay on Agarose Plates (Created page with "1. Measure the density of an overnight M. xanthus culture using the spectrophotometer. Before spinning and prepping your samples, calculate the volume of TPM starvation buffer that you will use to bring your overnight culture to a concentration of 5x10^9 cells/mL that we typically use for starvation assays. If you take 1mL of your overnight culture at the density that you calculated, you can use the C1V1=C2V2 calculation. (1mL)(O/N culture cells/mL) = (5x10^9 cells/m...")