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• 21:37, 11 May 2023 Dcapcha talk contribs created page Western Blot (Created page with "==Goal== * To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host. ==Protocol== ===Day 1=== ====Induction and Overnight Incubation of Bacterial Culture==== '''Day 2: Obtaining Supernatant and Lysate Samples from Bacterial Culture''' '''Da...")