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• 10:03, 31 March 2023 Jcomstock talk contribs created page EcoRI digest of plasmid (Created page with "Since the TOPO pcr2.1 plasmid has EcoRI cut sites on either side of the PCR insert, you can perform a restriction digest with EcoRI enzyme to check the size of the insert after plasmid extraction. This ensures that the plasmid you extracted from the E. coli liquid culture is consistent with your colony PCR results. (Remember that your culture could become contaminated with something else between colony PCR and plasmid extraction, so this digest serves as a verification b...")