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• 09:28, 31 March 2023 Jcomstock talk contribs created page Plasmid Isolation with BioBasic Miniprep Kit (Created page with "1. On the day before your minipreps you will use sterile technique near a Bunsen burner to set up 3ml of LB culture with 50ug/ml of kanamycin (or other appropriate antibiotic) (add 5ul of the 50mg/ml stock solution) for each colony of interest. Using a sterile P200 pipette tip touch one colony from your patch plate and then drop it into one culture tube. Be careful to record which culture corresponds to which patch on your patch plate. Place cultures (with lids at the lo...")