All public logs

Combined display of all available logs of Microbial Ecology and Evolution Lab Wiki. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 12:00, 29 March 2023 Tommy talk contribs created page Transform competent E. coli cells (Created page with "Transform competent E. coli cells 1.Prepare for transformation by ensuring the following: Water bath is set to 42°C Vial of SOC medium is warmed to room temperature X-gal/Kan plates are warmed to 37°C One Shot® Chemically Competent E. coli are thawed on ice Tubes of competent cells will be shaken horizontally which means cells will come into contact with the cap of the tube. To maintain good sterile technique and prevent contamination, do not touch the insi...")