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• 10:33, 21 March 2023 Jcomstock talk contribs created page PCR Amplification from Genome (Created page with "1. Extract template M. xanthus DNA with genomic DNA miniprep kit or other protocol. Dilute 1:10 to use for this PCR protocol (you will receive already diluted DNA). Note: check freezer first we probably have some isolated gDNA. 2. Prepare PCR reaction master mix. Calculate for the number of reactions plus 1. You will run your experimentalsamples w ith M. xanthus template DNA and your designed primers, a positive control with M. xanthus template DNA and 16s rRNA primer...")