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• 13:48, 9 March 2023 Xiwei Huang talk contribs created page Preparing the Soft Agar (Created page with "1. The soft agar should be prepared first, likely at least an hour before you pour a. Make sure the water bath has enough water and is warming to 42C b. You will use about 4 mL of soft agar per plate, calculate total volume of agar needed accordingly i. Always prep at least one extra plate c. Loosen the cap of the soft agar bottle and microwave the bottle of agar until it is fully liquid: i. Check for bubbling about every 15 seconds ii.You will kno...")