Western Blot
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Goal
To extract protein samples from E. coli, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of E. coli.
Protocol
Day 1
Induction and Overnight Incubation of E. coli Culture
1. Grab however many erlenmeyer flasks you need for the experiment (including one for the negative control)
2.
3.