Zone of Inhibition Assay
Jump to navigation
Jump to search
The night/ day before create liquid cultures of the two bacteria that wish to work with
In the hood:
- Heat bock set to 45 degrees
- Vortexer
- 10ul, 200ul, and 1000ul pipettor
- Auto pipettor
- 10ul, 200ul and 100ul pipet tips
- 24 well plate
Create a beaker of verkon
- Fill a beaker with 500ml of water
- Add two ‘john dear’ spoon full of Verkon
Zone of Inhibition
- Set the heat block to 45 degrees
- Add 8 empty 13mm tubes with caps to the block
- Warm up the BHI agar in the science microwave
# Start at power level 6
# Watch --> if starts to boil lower the power level # Should take about 10 min
- When all melted lave to cool for a little so it is not boiling
# To cool a little faster put in a cool water bath
- Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles)
Note: multiple plate can be made in advance, but don’t leave for longer then a week
- Label plate