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Revision as of 14:59, 4 September 2019 by PreDec2022>MadeleineGallic
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Storage Buffer Preparation for R5(2)-mCh-FL-BST
final concentration (with pH 7.1):
storage buffer recipe
|
Amount
|
Chemical
|
30 mM
|
KCl
|
2.5 mM
|
TRIS-HCl
|
0.025 mM
|
EDTA
|
1 mM
|
DTT
|
0.1%
|
Triton X-100
|
procedure for 250 mL:
- weigh out the following powders: KCl, TRIS, EDTA, DTT
measured mass
|
|
Desired Amount
|
KCL
|
559.14 mg
|
TRIS
|
75.71 mg
|
EDTA
|
1.83 mg
|
DTT
|
38.56 mg
|
- add all chemicals directly to a 250 mL pyrex container
- add 250 mL H2O to container
- add 250 μL Triton X-100
- adjust pH to 7.25
Calculations:
Quantity calculator
|
|
g/mol
|
desired mM
|
desired mL
|
required mg
|
KCL
|
74.5513
|
30
|
250
|
559.13475
|
TRIS
|
121.14
|
2.5
|
250
|
75.7125
|
EDTA
|
292.24
|
0.025
|
250
|
1.8265
|
DTT
|
154.253
|
1
|
250
|
38.56325
|