Reagent Recipes
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Reagent Information
| Reagent | Abbreviation | Stock Solution
Concentration |
Solvent | Stock Concentration to
Working Concentration |
Notes |
|---|---|---|---|---|---|
| Bromo-chloro-indolyl-galactopyranoside | X-gal | 40mg/ml | Dimethylformamide (DMF) | 500x | Use DMF in a chemical hood |
| Isopropyl β-D-1-thiogalactopyranoside | IPTG | 0.1M | H2O | 1000x | Light sensitive. Molecular weight: 238.31 g/mol |
Glycerol for freezing aliquots
We use sterile 80% glycerol in water. We dilute this 1:3 (400ul 80% glycerol; 1200ul culture) for creating freezer stocks with a final glycerol concentration of 20%.