Streptococcus mutans Growth
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Generic Tips
- Strains can grow on Tryptic Soy Broth plates (TSB) or TSB + Blood plates. There is no hemolysis on blood plates.
- Strains take 2 days to grow on plates from the freezer; please plan accordingly!
- Shaking is not needed. Notice that these cells have a more granular appearance, as they form tight clusters of cells.
- Overnight cultures in BHI begin to die off; please do not plan on using them for more than the next day, if at all.
- Less death is evident in SMSDM (S. mutans Semi-defined Media); same for BHI plates; we notice few cells surviving after 48 hours.
SMSDM
Base Medium
Base Media 1.11x concentrationCompound | Amount | Final Concentration |
---|---|---|
K2HPO4 | 5.61g | 58mM |
KH2PO4 | 1.13g | 15mM |
(NH4)2SO4 | 734mg | 10mM |
NaCl | 1.14g | 35mM |
Casamino Acids | 1.10g | 0.2% |
- Fill to 450ml
- pH to 7.4