Blue Tongue

Maan S, Maan NS, Batra K, Kumar A, Gupta A, Rao PP, Hemadri D, Reddy YN, Guimera M, Belaganahalli MN, Mertens PP. “Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India” (ask for access to this paper)

• Reverse-transcription-loop-mediated-isothermal-amplification (RT-LAMP) assay were created to detect eastern or western topotype of BTV. They created primers that recognized 6-distinct sequences of BTV genome segment 1. These assays were able to detect BTV RNA in all positive isolates tested. The sensitivity of RT-LAMP is similar to the sensitivity of RT-PCR but higher than conventional RT-PCR. Could not detect other local viruses that cause symptoms similar to BTV.

Jung-Yong Yeh, Ji-Hye Lee, Hyun-Ji Seo, Jee-Yong Park, Jin-San Moon, In-Soo Cho, In-Soo Choi,Seung-Yong Park, Chang-Seon Song, Joong-Bok Lee (2011). [Simultaneous Detection of Rift Valley Fever, Bluetongue, Rinderpest, and Peste des Petits Ruminants Viruses by a Single-Tube Multiplex Reverse Transcriptase-PCR Assay Using a Dual-Priming Oligonucleotide System]