Streptococcus mutans Growth: Difference between revisions
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= Generic Tips = | |||
* Strains can grow on Tryptic Soy Broth plates (TSB) or TSB + Blood plates. There is no hemolysis on blood plates. | * Strains can grow on Tryptic Soy Broth plates (TSB) or TSB + Blood plates. There is no hemolysis on blood plates. | ||
* Strains take 2 days to grow on plates from the freezer; please plan accordingly! | * Strains take 2 days to grow on plates from the freezer; please plan accordingly! | ||
* Shaking is not needed. Notice that these cells have a more granular appearance, as they form tight clusters of cells. | * Shaking is not needed. Notice that these cells have a more granular appearance, as they form tight clusters of cells. | ||
* Overnight cultures in BHI begin to die off; please do not plan on using them for more than the next day. | * Overnight cultures in BHI begin to die off; please do not plan on using them for more than the next day, if at all. | ||
* Less death is evident in SMSDM (S. mutans Semi-defined Media); same for BHI plates; we notice few cells surviving after 48 hours. | |||
= SMSDM = | |||
== Base Medium == | |||
{| class="wikitable" style="margin:auto" | |||
Base Media 1.11x concentration | |||
! Compound !! Amount !! Final Concentration | |||
|- | |||
| K2HPO4 || 5.61g || 58mM | |||
|- | |||
| KH2PO4 || 1.13g || 15mM | |||
|- | |||
| (NH4)2SO4|| 734mg || 10mM | |||
|- | |||
| NaCl || 1.14g || 35mM | |||
|- | |||
| Casamino Acids || 1.10g || 0.2% | |||
|} | |||
* Fill to 450ml | |||
* pH to 7.4 | |||
Revision as of 20:41, 16 October 2023
Generic Tips
- Strains can grow on Tryptic Soy Broth plates (TSB) or TSB + Blood plates. There is no hemolysis on blood plates.
- Strains take 2 days to grow on plates from the freezer; please plan accordingly!
- Shaking is not needed. Notice that these cells have a more granular appearance, as they form tight clusters of cells.
- Overnight cultures in BHI begin to die off; please do not plan on using them for more than the next day, if at all.
- Less death is evident in SMSDM (S. mutans Semi-defined Media); same for BHI plates; we notice few cells surviving after 48 hours.
SMSDM
Base Medium
Base Media 1.11x concentration| Compound | Amount | Final Concentration |
|---|---|---|
| K2HPO4 | 5.61g | 58mM |
| KH2PO4 | 1.13g | 15mM |
| (NH4)2SO4 | 734mg | 10mM |
| NaCl | 1.14g | 35mM |
| Casamino Acids | 1.10g | 0.2% |
- Fill to 450ml
- pH to 7.4