Western Blot: Difference between revisions
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==Goal== | ==Goal== | ||
* | * | ||
To extract protein samples from | To extract protein samples from <i>E. coli</i>, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of <i>E. coli</i>. | ||
==Protocol== | ==Protocol== | ||
===<u>Day 1</u>=== | ===<u>Day 1</u>=== | ||
====Induction and Overnight Incubation of | ====Induction and Overnight Incubation of <i>E. coli</i> Culture==== | ||
1. Grab however many erlenmeyer flasks you need for the experiment (including one for the negative control) | |||
2. | |||
3. | |||
===<u>Day 2</u>=== | ===<u>Day 2</u>=== | ||
Revision as of 21:59, 11 May 2023
Goal
To extract protein samples from E. coli, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of E. coli.
Protocol
Day 1
Induction and Overnight Incubation of E. coli Culture
1. Grab however many erlenmeyer flasks you need for the experiment (including one for the negative control)
2.
3.